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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Cell Signal. 2014 Jun 3;26(10):2276–2283. doi: 10.1016/j.cellsig.2014.05.018

Figure 3.

Figure 3

EGF activates multiple signaling pathways in HKC. (A) Cells were incubated with EGF (25 ng/ml) for the indicated intervals. Total cell lysates were analyzed by western blot using specific antibodies against phosphorylated forms of STAT1, STAT3, ERK, c-Jun, AKT (pSTAT1, pSTAT3, pERK, p-c-Jun, pAKT) and their corresponding non-phosphorylated forms. β-actin was used as a loading control. (B) Densitometric analysis of pSTAT1, pSTAT3, pERK, p-c-Jun and pAKT was normalized to β-actin and expressed as fold change over vehicle alone. *p<0.05, **p<0.005 comparing with vehicle control (n=3).