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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Ultrasound Med Biol. 2014 Jul 9;40(9):2172–2182. doi: 10.1016/j.ultrasmedbio.2014.03.018

Fig. 2. Cytoplasmic Ca2+ changes in HUVECs due to HFUMS.

Fig. 2

(a) Fluorescence images obtained before (left), during (middle), and after (right) HFUMS (input voltage: 15.8 Vpp, PRF: 1 kHz, and duty cycle: 1 %) (b) Ca2+ changes in HUVECs over times. HFUM was switched on at 60 s and off at 240 s (c) Quantitative analysis of calcium elevation. The CRI values for HUVECs without (control) (n=21) and with (n=21) HFUMS were calculated with the program described previously. The scale bar indicates 10 μm.