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Pluripotency and germ layer markers of ES cells differentiated in substrates of varying and extreme fibrinogen concentration but same cross-linking ratio of 1X, in (A) 2-dimensional and (B) 3-dimensional culture. Significant upregulation of endoderm markers was found when cells were differentiated on gels obtained at lower fibrinogen concentration. * p<0.05 compared to highest stiffness group.
