Figure 5.

Histopathologic analysis of tissues for assessment of drug-induced toxicity. (A) Animals received three daily IV doses of the FAP-activated ERGETGP-S12ADT prodrug and tissues were harvested 20 days post-therapy for comparison to vehicle-treated controls. All tissues were examined in a blinded fashion and no differences could be detected between control and treated animals. All tissues were considered histopathologically normal. Three animals per group were examined and images are representative of a typical 10× field. (B) The only abnormal pathology observed was a single granuloma with mild inflammation in the lung of a single treated animal. Small granuloma (upper panel) along with moderate perivascular inflammation marked by the presence of lymphocytes and macrophages (lower panel). This event was considered unrelated to therapy as they only occurred in a single animal and no evidence of apoptotic cells or other drug-related mechanisms of action were observed. Images were taken at 20×.