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. 2014 Jun 5;5(8):569–579. doi: 10.1007/s13238-014-0073-9

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© The Author(s) 2014

Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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Aberrant mitosis and micronucleus formation in hPSCs. (A) Immunostaining of euploid HUES1 hESCs for AURORA A (a and b), AURORA B (c and d) and SURVIVIN (e, f). Nuclei are counterstained with DAPI. Panels (a and b) show an example of a multipolar division with multipolar spindles. Note that AURORA A (green staining) is localized to all three spindle poles (white arrows in b). Panels c–f show an example of a chromosomal bridge (white arrows in c and e). Note that AURORA B (d, green staining) and SURVIVIN (f, green staining) are concentrated at the cleavage furrow and the lagging kinetochore (white arrows in d and e). (B) Micronucleus formation (white arrow) in H9 hESCs after inhibition of AURORA B with 50 nm of AZD1152 for 24 h and release from the inhibitor for 24 h. Micronucleus stained positive for γH2AX, a marker of DNA damage (green)