Table 1.
Molecular weights and oligomerization states of Uch37 and its complex with Rpn13C estimated by different methods
| Protein | MW (kDa) calculated from primary sequence | MW estimated from different methods | Oligomerization status | ||
|---|---|---|---|---|---|
| Method | Concentration (mg/mL) | MW (kDa) | |||
| Uch37 | 37.0 | SEC | 0.4 | 50.7 | Monomer, dimer |
| 1.6 | 58.0 | Monomer, dimer | |||
| 10.0 | 128.9 | Trimer, tetramer | |||
| AUC | ~1.0 | 28.8 and 50.1 | Monomer, dimer | ||
| SAXS | 1.3 | 117.7 | Dimer, trimer, tetramer | ||
| Uch37-Rpn13C | 52.5 | SEC | 1.2 | 66.4 | Monomer |
| 8.0 | 66.8 | Monomer | |||
| AUC | ~1.0 | 56.4 | Monomer | ||
| SAXS | 1.3 | 64.6 | Monomer | ||
| Uch37ΔHb,Hc,KEKE | 29.1 | SEC | >7.0 | 34.3 | Monomer |
| Uch37ΔHc,KEKE | 33.3 | SEC | >4.0 | 38.0 | Monomer |
| AUC | ~1.0 | 30.1 | Monomer | ||
| Uch37ΔKEKE | 34.9 | SEC | >1.5 | 41.9 | Monomer |
SEC, size exclusion chromatography; AUC, analytical ultracentrifugation; SAXS, small angle X-ray scattering