Table 2.
Protein sample | Rg (Å)a | Dmax (Å)b | VPorod (Å3)c | MWd (kDa) | Oligomeric state |
---|---|---|---|---|---|
Uch37 | 40.74 ± 0.14 | 120–130 | 197,367 ± 12,025 | 117.7 ± 1.1 | Dimer and tetramer |
Uch37-Rpn13C | 29.73 ± 0.17 | 85–90 | 90,879 ± 1403 | 64.6 ± 1.0 | Heterodimer |
aThe real-space Rg was estimated by calculating P(r) using GNOM
bDmax was estimated by calculating P(r) using GNOM
cThe Porod volume was calculated using PRIMUS
dThe molecular weights (MWs) were predicted using the web server http://www.if.sc.usp.br/~saxs/saxsmow.html) (Fischer et al., 2010)
eValues are the average of three independent experiments; error bars represent the standard error of the mean