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. 2014 Apr 22;5(8):616–630. doi: 10.1007/s13238-014-0046-z

Table 2.

Parameters derived from SAXS curves

Protein sample Rg (Å)a Dmax (Å)b VPorod3)c MWd (kDa) Oligomeric state
Uch37 40.74 ± 0.14 120–130 197,367 ± 12,025 117.7 ± 1.1 Dimer and tetramer
Uch37-Rpn13C 29.73 ± 0.17 85–90 90,879 ± 1403 64.6 ± 1.0 Heterodimer

aThe real-space Rg was estimated by calculating P(r) using GNOM

bDmax was estimated by calculating P(r) using GNOM

cThe Porod volume was calculated using PRIMUS

dThe molecular weights (MWs) were predicted using the web server http://www.if.sc.usp.br/~saxs/saxsmow.html) (Fischer et al., 2010)

eValues are the average of three independent experiments; error bars represent the standard error of the mean