Table 1.
Patient characteristics, histology and follicle viability. Characteristics of patients at the time of ovarian tissue cryopreservation and results of the Calcein follicle viability staining and histology (‘-‘no follicles were observed in the histologically evaluated biopsy). N = patient number. HL = Hodgkin’s lymphoma. NHL = Non-Hodgkin’s lymphoma. Transport temp = Temperature measured in the transport box at arrival in Bonn (°C). (D) = Degenerated follicle as observed during histological examination. IQR = Interquartile range
| N | Age | Cancer type | AMH level (μg/L) | Transport Temp (ºC) | Viable follicle count (7 mm3 cortex tissue) | Histology (14 mm2 cortex tissue) | ||
|---|---|---|---|---|---|---|---|---|
| Before cryopreservation | After cryopreservation | Before cryopreservation | After cryopreservation | |||||
| 1 | 24 | Rectal | 1,6 | 5 | 65 | 62 | 2 primordial (1 D) | 1 primordial |
| 2 | 31 | Breast | 0,6 | 4,5 | 47 | 39 | 1 primordial | - |
| 3 | 29 | Cervix | no sample | 4 | 47 | 28 | - | 4 primordial |
| 4 | 21 | HL | no sample | 4 | 173 | 291 | 11 primordial, 4 primary, 6 secondary | 1 primordial (D), 8 primary, |
| 5 | 33 | HL | 1,9 | 6 | 102 | 113 | 7 primordial (1 D) | 2 primordial (D), 1 primary (D) |
| 6 | 39 | Breast | 1,6 | 4 | 32 | 24 | 5 primordial (2 D) | - |
| 7 | 21 | HL | 2,9 | 6,5 | 248 | 310 | 31 primordial, 2 primary | - |
| 8 | 20 | HL | 3,6 | 5,5 | 231 | 183 | 48 primordial, 4 primary | - |
| 9 | 27 | HL | 2,1 | 5,5 | 128 | 133 | - | - |
| 10 | 23 | Chondrosarcoma | 1,8 | 5,5 | 128 | 46 | - | - |
| 11 | 33 | Breast | 1,8 | 4,5 | 93 | 135 | 6 primordial, 6 primary, 1 secondary | 18 primordial, 1 primary |
| 12 | 26 | Cervix | no sample | 6,5 | 74 | 24 | - | - |
| 13 | 29 | Breast | 2,1 | 4,5 | 83 | 220 | 3 primordial | 3 primordial, 1 antral |
| 14 | 21 | NHL | 1,1 | 5 | 291 | 91 | 19 primordial, 5 primary | - |
| 15 | 35 | Breast | 0,6 | 7 | 38 | 40 | − | 1 secondary |
| 16 | 31 | Breast | no sample | 6 | 286 | 350 | − | − |
| 17 | 21 | HL | 2,5 | 5,5 | 400 | 233 | − | − |
| 18 | 31 | Breast | 1,4 | 2 | 53 | 160 | − | − |
| 19 | 24 | HL | 1,8 | 6,5 | 129 | 150 | − | 5 primordial (2 D) |
| 20 | 36 | Breast | 1,9 | 10 | 18 | 27 | − | − |
| 21 | 20 | B-cell lymphoma | 2,5 | 5,5 | 180 | 200 | − | − |
| 22 | 35 | Breast | 1,9 | 5,5 | 10 | 13 | 1 primordial (D) | − |
| 23 | 31 | Breast | 0,8 | 5,0 | 34 | 46 | − | − |
| 24 | 34 | Breast | 2,9 | 5,5 | 56 | 76 | − | 2 primordial (1 D) |
| 25 | 18 | Breast | 2,1 | 6,0 | 258 | 93 | − | 9 primordial (6 D) |
| Median (IQR) | Total | |||||||
| 29 (21–33) | 5.5 (4.5–6.0) | 93 (47–206) | 93 (40–192) | 162 follicles of which 5 degenerated (97% intact) primordial follicles: 112 (5 degenerated; 96% intact) |
57 follicles of which 13 degenerated (77% intact) primordial follicles: 45 (12 degenerated; 73% intact) |
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