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. Author manuscript; available in PMC: 2015 Feb 7.
Published in final edited form as: Nat Commun. 2014 Aug 7;5:4575. doi: 10.1038/ncomms5575

Figure 3.

Figure 3

Up-regulation of Th9 cell polarization by NO is IL-2-dependent. C57BL6 CD4+ T cells were cultured under Th9 polarizing conditions ± NO. (a) Il2 mRNA was determined by qPCR on day 2. (b) IL-9 and IL-2-producing cells were analyzed by FACS on day 4. (c) IL-2 in cell culture supernatants determined by ELISA. (d) IL-9 concentrations in the supernatant of Th9 polarized cells cultured in the presence of αIL-2 antibodies (20 μg/ml) were determined on day 5. Data are representative of at least 3 independent experiments; Mean ± SEM, * P<0.05, *** P< 0.001 using a 2-way ANOVA.