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. Author manuscript; available in PMC: 2015 Feb 7.
Published in final edited form as: Nat Commun. 2014 Aug 7;5:4575. doi: 10.1038/ncomms5575

Figure 6.

Figure 6

NO enhances Th9 polarization via nitrosylation of MDM2-p53 complex. (a) BALB/c CD4+ T cells were polarized under Th9 conditions ± 100 μM NOC-18. p53/MDM2 complex in the cell extracts were determined by ELISA. (b) CD4+ T cells were polarized as above in the presence of cysteine (1 mM) or ascorbate (300 μM) for 24 h and the cell extracts analyzed for p53/MDM2 complex. Percentage of IL-9+p53+ T cells on day 4 (c,), IL-9+IL-2+ T cells on day 4 (d), IL-9+pSTAT5+ T cells on day 5 (e), and IL-9+IRF4+ T cells (f) on day 4 analyzed by FACS. Data are representative or pooled of at least 3 experiments; mean ± SEM, n=3-5, *P<0.05, **P<0.01, ***P<0.001 using a 2-way ANOVA.