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. 2014 Aug 4;14:285. doi: 10.1186/1472-6882-14-285

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© Fang et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of EA on p-CREB DNA binding activity in L4-6 ipsilateral spinal cord dorsal horn. (A). EMSA experiment showing binding to CREB probe in nuclear extracts prepared from control and model, EA, and sham EA groups at 6 and 49 h after CFA injection. The binding of CREB in the control group was considered for comparison (lane 2). EA regulated CREB protein binding activity at 49 after CFA-treated (lane 7). (B). EMSA experiment showing the binding of proteins present in the nuclear extracts prepared from the model group at 49 h after CFA injection. Specificity of the DNA-protein interaction was assessed by competition EMSA using a 100× molar excess of unlabeled doubled-stranded oligonucleotide CREB probe (lane 3), unlabeled mutant doubled-stranded oligonucleotide CREB probe (lane 4) and biotin-labeled mutant doubled-stranded oligonucleotide CREB probe (lane 5).