Figure 2.

(a) Photomicrograph of 5-bromo-2-deoxyuridine (BrdU) immunopositive cells (new born neurons) in DG in the hippocampus of control and OVX mice with 17β-E2 or α-ZAL treatment. Fewer BrdU-labeled cells were observed in OVX mice compared to control. 17β-E2 and α-ZAL-treated mice expressed higher BrdU⁺ cells compared to OVX animal. Magnification ×40. (b) Quantification of BrdU immunolabeling cells in DG. n = 4 in each group and the result indicated at least three independent experiments in each animal. **P < 0.01 versus control group; ^## P < 0.01 versus OVX group.