Fig. 1.

LPS induces IL-6 production and EOLA1 in human umbilical vein endothelial cells (HUVEC). a HUVEC were treated with LPS (100 ng/ml) for various periods of time 0, 4, 8, 16 h and the supernatants were collected and assayed for IL-6 production by ELISA. b HUVEC were treated with various doses of LPS 0, 1, 10, 50 and 100 ng/ml for 16 h and he supernatants were collected and assayed for IL-6 production by ELISA. Data represent the mean ± S.D of three independent experiments. c LPS induces EOLA1 expression in HUVEC. Cells were stimulated with LPS (100 ng/ml) for the indicated periods of time. The lysates were analyzed by Western blotting with antibodies to EOLA1. Data show one representative experiment out of three independent experiments