Fig. 2.

Inhibition of EOLA1 with siRNA inhibits LPS-induced IL-6 production and induces apotosis in HUVEC. a HMVEC were transfected with control or EOLA1 siRNA (200 nM) for 48 h at 37 °C. The cells were lysed and analyzed by Western blotting with EOLA1 antibody. b HMVEC were transfected with control or EOLA1 siRNA (200 nM) for 24 h at 37 °C and then cultured with or without LPS (100 ng/ml) and the concentration of IL-6 in the culture supernatants was determined 16 h after stimulation. Data represent the mean ± S.D of three independent experiments. (C) HMVEC cells pre-treated with EOLA1 siRNA were stimulated with LPS (100 ng/ml) for 16 h. The supernatants were then tested for apoptosis. (**P < 0.01, *P < 0.05)