Table 1. Coefficient of variation of chromosome peaks of derivative 11 and 15 from B19580t (11;15) lymphoblastoid cell line.
der 11 |
der 15 |
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COEFFICIENT OF VARIATION |
COEFFICIENT OF VARIATION |
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LASER POWER (mW) | HOECHST (% DECREASE IN CV OVER LASER POWER AT 25 mW) | CA3 (% DECREASE IN CV OVER LASER POWER AT 25 mW) | HOECHST (% DECREASE IN CV OVER LASER POWER AT 25 mW) | CA3 (% DECREASE IN CV OVER LASER POWER AT 25 mW) |
25 | 2.94 | 3.19 | 1.73 | 3.12 |
50 | 2.78 (−5.4%) | 2.96 (−7.2%) | 1.53 (−11.6%) | 2.85 (−8.7%) |
300 | 2.35 (−20%) | 2.77 (−13.2%) | 1.46 (−15.6%) | 2.48 (−20.5%) |
Chromosomes were prepared using a polyamine isolation method and analyzed with laser power intensities varied from 25 mW to 300 mW.