Figure 1.

PELP1 is phosphorylated at Ser-1033 residue upon various genomic insults. (a) MCF7 cells were metabolically labeled with ³²P-orthophosphate, and treated with radiation (10 Gy, 30 min). PELP1 phosphorylation was determined by immunoprecipitation followed by autoradiography. (b) MCF7 cells were treated with UV radiation (100 mJ/s), PELP1 was immunoprecipitated and treated with or without λ-phosphatase. Status of PELP1 phosphorylation was visualized by western blotting using PELP1 Ser1033 phospho-antibody. (c) ZR75 cells expressing GFP, GFP-PELP1, or GFP-PELP1-S1033A MT or PELP1-shRNA were treated with or without UV radiation, followed by western blotting with PELP1 Ser1033 phospho-antibody. (d) ZR75 cells were exposed to different doses of γ-radiation, followed by western blotting with PELP1 Ser1033 phospho-antibody. (e) ZR75 and ZR75-PELP1-shRNA were exposed to UV radiation, followed by western blotting with PELP1 Ser1033 phospho-antibody. (f) 293T cells were exposed to genotoxic stress by H₂O₂ (30 min) or etoposide (50 μM, 1 h) followed by western blot analysis with PELP1 Ser1033 phospho-antibody