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. 2014 May 2;21(9):1409–1418. doi: 10.1038/cdd.2014.55

Figure 2.

Figure 2

ATM, ATR and DNAPKc kinases can phosphorylate PELP1 at S1033 upon DNA damage. (a) ATM-proficient, human fibroblasts GM00637 and ATM-deficient fibroblast GM05849 cells were treated with or without etoposide (50 μM,1 h) and the status of PELP1 phosphorylation at S1033 was analyzed by western blotting. (b) Human glioma cells M059K and M059J, proficient and deficient in DNAPKc, respectively, were treated with or without etoposide (50 μM, 1 h) alone or in combination with KU55933 and the status of PELP1 phosphorylation at S1033 was analyzed by western blotting. (c and d) Human skin fibroblast GM00200 and GM18366, proficient and deficient in ATR functions, respectively, (c) or ATM proficient GM00637 and ATM-deficient cells GM05849 (d) were irradiated with UVC and the status of PELP1 phosphorylation at S1033 was analyzed by western blotting. (e) ZR75 cells were pre-treated with increasing doses of caffeine and irradiated with 100 mJ/s UVC. Status of PELP1 S1033 phosphorylation was analyzed by western blotting