Figure 6.

p73 is required for p53 stabilization under AICAR but not under cisplatin treatment. (a) HCT116 cells were either untreated or incubated with 0.5 mM AICAR for 24 h. CHX (20 μg/ml) was then added, and cells were collected at different time points and analyzed by immunoblotting to measure p53 protein half-life. Actin serves as a loading control. (b) HCT116 cells stably expressing a non-silencing cassette (control) or an shRNA directed against p73 (p73 KD) were treated with 0.5 mM AICAR for 24 h. CHX (20 μg/ml) was then added, and cells were collected at different time points to measure p53 protein half-life. Actin serves as a loading control. (c) Same as in (b), only here cells were treated with 25 μM cisplatin for 24 h