Figure 3.

Comparison of commonly used and HyCoSuL-derived caspases substrates. Six ACC substrates with commercially available sequences were tested toward six recombinant human caspases (left panels). For each substrate, all three kinetic parameters were determined (see Supplementary Data section). Here we present only k_cat/K_M values ( × 10⁴ M⁻¹s⁻¹). Comparison with the most selective substrates derived from HyCoSuL screening reveals the utility of this approach in developing highly selective tetrapeptide substrates