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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Nat Methods. 2014 Jun 15;11(8):861–867. doi: 10.1038/nmeth.2998

Figure 1.

Figure 1

Cytosolic delivery of dfTAT in live cells is efficient and exceeds its monomeric counterparts. (a) Cellular localization of acfTAT and dfTAT assessed by fluorescence microscopy. Cells were incubated for 1 h with either acfTAT (20 μM) or dfTAT (5 μM), washed, and imaged with a 100x objective. Monochrome images represent the emission of TMR at 560 nm. Scale bars, 10 μm. (b) Comparison of the cytosolic delivery efficiency of acfTAT, fTAT, and dfTAT. Cells were incubated with acfTAT, fTAT, and dfTAT (1-20 μM) for 1 h. The number of cells with detectable cytosolic and nuclear fluorescence distribution in microcopy images was counted and divided by the total number of cells present (%) (1,000 cells/experiment). (c) dfTAT overall uptake in HeLa cells as a function of the concentration of dfTAT present in the incubation media. Cells were incubated with dfTAT (1-10 μM) for 1h and relative uptake was assessed quantitatively by measuring the bulk fluorescence of cell lysates (300,000 cells/experiment). The data shown in b and c represent the mean of triplicate experiments and the corresponding standard deviations.