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. Author manuscript; available in PMC: 2015 Jul 31.
Published in final edited form as: Cell. 2014 Jul 31;158(3):593–606. doi: 10.1016/j.cell.2014.05.049

Figure 6. LUNAR1 is able to stimulate transcription of a reporter gene.

Figure 6

(A) Illustration describing the BoxB Gal4-λN RNA tethering system used. (B) ChIP assay for Gal4-DBD at the reporter gene promoter. (C) Luciferase reporter activity in experiments where BoxB tagged LUNAR1 (blue) or HOTTIP (yellow) were co-transfected with Gal4-λN. (D) qPCR following ASO knockdown of LUNAR1 in luciferase assay. (E) Reporter assay showing relative reporter gene activity when BoxB-LUNAR1 was co-transfected with either non-targeting (blue) or LUNAR1-specific (yellow) ASOs. Error bars represent SEM of at least 3 experiments. * indicates p-value < 0.05. ** indicates p-value < 0.01.