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. 2014 Aug 13;4:6048. doi: 10.1038/srep06048

Figure 2. Sequence abnormalities in B117H Ara-C resistant cells verified by Sanger sequencing.

Figure 2

(a) RNA-seq reads were mapped to the NCBI reference genome. Visualization of Dck expression using IGV. (b) Transcripts were assembled independently of a reference transcriptome using Cufflinks, then mapped to the mm9 mouse genome using Cuffcompare, and the resulting gtf file was visualized by IGV. (c) Sanger sequencing of DNA in B117H cells identified an 878 nt deletion spanning the splice acceptor of intron 6 and the translated portion of exon 7. Sanger sequencing of RNA verified a transcript matching the configuration identified by TopHat and IGV.