Table 2. Protein modifying missense mutations found in the B117H and B140H cells, but not found in the respective parental cell lines. Predicted detrimental mutations in bold.

		Variant				Seq.	Protein
Cell Line	Gene	Position	Ref.	Var.	Prevalence	Depth	Change
B117H	Commd5	chr15:76730961	T	G	0.37	38	L43V
	Dram1	chr10:87803336	G	A	0.53	17	V91I
	Kdm3b	chr18:34987086	T	C	0.40	30	S1157P
	Kdm5c	chrX:148705903	G	T	0.33	39	V1222L
	Pigt	chr2:164328088	C	T	0.47	108	P386S
	Prkacb	chr3:146409579	G	T	0.44	94	E209D
	Pus7l	chr15:94358455	C	T	0.37	19	R499C
	Pyroxd1	chr6:142303201	A	G	0.43	7	N179S
	Rasgrp2	chr19:6407273	G	T	0.41	99	S349I
	Susd1	chr4:59328600	T	A	0.35	72	S748T
	Vps33b	chr7:87432008	G	A	0.37	27	D414N
B140H	Atpbd4	chr2:114470474	C	G	0.45	33	L99V
	Chd7	chr4:8678934	A	G	0.50	6	N95D
	Map3k11	chr19:5700807	G	A	0.38	52	M684I
	Mettl16	chr11:74609506	G	A	0.34	67	R203Q
	Pbrm1	chr14:31863259	A	C	0.37	62	S375R
	Rnf149	chr1:39609321	G	A	0.49	142	C394Y
	Smarca4	chr9:21445509	G	A	0.49	164	E525K
	Spag5	chr11:78118221	C	T	0.42	73	S284L
	Usp34	chr11:23321104	C	G	0.50	38	L1812V
	Zfp668	chr7:135009900	G	A	0.43	37	R542Q