Table 2.

Summary of parameters obtained by fitting the experimental FITC–RNase desorption kinetics to the two adsorbed protein populations model (Eq. (4))^a

Parameter	1	2	3	4	5	1	2	3	4	5
θ	0°	23°	34°	41°	46°	0°	23°	34°	41°	46°
	Ionic strength, 0.0185 M; pH 7.0					Ionic strength, 0.1185 M; pH 7.0
$k_{\text{off}}^{\mathrm{a}}({\mathrm{s}}^{-1})$	0.0157	0.0144	0.0147	0.0143	0.0136	0.0314	0.0299	0.0291	0.0294	0.0277
$k_{\text{off}}^{\mathrm{b}}({\mathrm{s}}^{-1})$	0.000684	0.000658	0.000655	0.000602	0.000558	0.00127	0.00111	0.00093	0.00081	0.00064
${\Gamma }_0^{\mathrm{a}}(\text{counts }{\mathrm{s}}^{-1})$ b	2036	1840	1832	1786	1969	3596	3121	2865	2668	2637
${\Gamma }_0^{\mathrm{b}}(\text{counts }{\mathrm{s}}^{-1})$ b	2547	2210	2299	2324	2591	1227	1126	1238	1381	1724
$\text{ln }2/k_{\text{off}}^{\mathrm{a}}(\text{min})$	0.7364	0.8010	0.7847	0.8070	0.8515	0.368	0.386	0.397	0.394	0.417
$\text{ln }2/k_{\text{off}}^{\mathrm{b}}(\text{min})$	16.88	17.55	17.65	19.19	20.71	8.26	9.45	11.28	12.94	17.66
${\Gamma }_0^{\mathrm{a}}/({\Gamma }_0^{\mathrm{a}}+{\Gamma }_0^{\mathrm{b}})$ c	0.444	0.454	0.444	0.435	0.432	0.746	0.735	0.698	0.659	0.605
${\Gamma }_0^{\mathrm{b}}/({\Gamma }_0^{\mathrm{a}}+{\Gamma }_0^{\mathrm{b}})$ d	0.556	0.546	0.557	0.566	0.568	0.254	0.265	0.302	0.341	0.395
R	0.9946	0.9945	0.9945	0.9933	0.9905	0.9945	0.9943	0.9929	0.9921	0.9557

^aWater contact angles are given for positions 1–5 indicated in Fig. 3.

^bFluorescence intensity at the onset of the desorption process.

^cFraction of total RNase population that will desorb with the faster rate.

^dFraction of total RNase population that will desorb with the slower rate.