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. 2014 Feb 7;28(8):1636–1646. doi: 10.1038/leu.2014.19

Figure 5.

Figure 5

MGCD0103-induced inhibition of autophagy is specific to primary CLL cells. (a) PBMCs from healthy donors (n=3) were incubated with or without MGCD0103 (0.5 or 3 μmol/l) and/or chloroquine (CQ; 20 μmol/l) for 24 h. (b, c) The effect of MGCD0103 on autophagy was assessed in cell lines. Cells were incubated with or without MGCD0103 (3 μmol/l) and/or Q-VD-OPh (10 μmol/l for CLL patient PBMCs and 20 μmol/l for cell lines) for 24 h (PBMC, JVM-2 and JVM-3) or 48 h (Mec-1 and MCF-7), and/or chloroquine (CQ; 40 μmol/l) for 24 h. The levels of indicated proteins were analyzed by immunoblotting using ACTB as a loading control. Representative blots from at least three independent experiments are shown in each panel.