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. Author manuscript; available in PMC: 2014 Aug 14.
Published in final edited form as: Nat Methods. 2013 Feb 17;10(3):246–248. doi: 10.1038/nmeth.2372

Figure 1. Unique identification of 20 loci on chromosome 19 by RNA FISH targeting introns in human foreskin fibroblasts.

Figure 1

a. Depiction of our pseudocoloring scheme for labeling the site of transcription by targeting gene introns with a series of labeled oligonucleotide probes. b. Using images from each fluorescence channel, we computationally identified the transcriptional activity and location of the 20 genes. Along with probes targeting the introns, we also included probes targeting Cyclin A2 mRNA to determine position in cell cycle and SUZ12 mRNA as a fiducial marker. The scale bar is 5μm long.