Table 1.
Comparison of known Wnt target genes to their expression levels in Gsk-3 DKO and S33A ESCs as determined by microarray.
| Gene | DKO | S33A |
|---|---|---|
| Brachyury | 162.5 | 41.7 |
| SP5 | 75.8 | 15.1 |
| Cdx1 | 16.5 | 82.5 |
| Axin-2 | 8.6 | 4.6 |
| Stra6 | 4.8 | 1.3 |
| LEF1 | 4 | −7 |
| Cyclin D | 3.1 | −3.5 |
| Pituitary tumor transforming gene (PTTG) | 2.2 | 1.8 |
| FGF18 | 2.1 | −2.9 |
| BMP4 | 1.8 | 1.2 |
| PPARδ | 1.6 | 1.1 |
| Endothelin-1 | 1.5 | 1.4 |
| Telomerase | 1.5 | 1.4 |
| LGR5/GPR49 | 1.5 | −1.2 |
| Jagged | 1.4 | 1 |
| sFRP-2 | 1.4 | −1.5 |
| CD44 | 1.3 | 1.4 |
| c-myc binding protein | 1.3 | −1.9 |
| Msl1 | 1.3 | 1.2 |
| Nitric oxide synthase 2 | 1.3 | 1.4 |
| Nkx2.2 | 1.3 | 1.2 |
| c-myc | 1.2 | −3.3 |
| Met | 1.2 | −1.2 |
| Claudin-1 | 1.1 | 1 |
| Id2 | 1.1 | 1.2 |
| FoxN1 | 1.1 | 1.1 |
| Gbx2 | 1.1 | −2.2 |
| MMP-7 | 1 | 1 |
| Osteoprotegerin | 1 | 1 |
| Wnt3a | 1 | 1.2 |
| Neurogenin 1 | 1 | 1.1 |
| Tiam1 | −1.1 | −1.2 |
| FGF20 | −1.1 | −1.4 |
| Cdx4 | −1.1 | 2.3 |
| Sox17 | −1.2 | −1.6 |
| Oct-4 | −1.2 | −1.3 |
| Runx2 | −1.3 | 1 |
| Sox2 | −1.3 | 1.4 |
| Frizzled 7 | −1.3 | −2.1 |
| Gastrin | −1.4 | 1.3 |
| Sox9 | −1.4 | −1.1 |
| CCN1/Cyr61 | −1.4 | −1.2 |
| Follistatin | −1.4 | 1.2 |
| NeuroD1 | −1.5 | −5.6 |
| c-jun | −1.6 | −2.2 |
| Nr-CAM | −1.6 | −1.5 |
| SALL4 | −1.6 | −2.3 |
| Nanog | −1.6 | 1.4 |
| FGF9 | −1.7 | 1 |
| Irx3 and Six3 | −1.8 | −1.4 |
| Cacna1γ | −1.8 | −1.8 |
| MMP2, MMP9 | −1.9 | 1.6 |
| LBH | −2 | 1.4 |
| Pitx2 | −2.1 | − 1.5 |
| ITF-2 | −2.3 | − 1.9 |
| Twist | −2.4 | −2 |
| Periostin | −2.4 | 1 |
| VEGF | −2.5 | 2.4 |
| Snail | −2.8 | − 1.5 |
| Tcf-1 (Hnf1a) | −3 | − 1.1 |
| n-myc | −3.4 | −3.4 |
| Gremlin | −3.5 | −3.5 |
| EGF receptor | −3.8 | − 1.5 |
| Delta-like 1 | −9.8 | −4.3 |
| WISP | −10 | − 1.1 |
The list of genes was found at The Wnt Homepage website (www.stanford.edu/group/nusselab/cgi-bin/wnt/target_genes). Genes whose expression increased more than twofold are shown in red, while genes whose expression decreased more than twofold are shown in green.