Table 4.
Expression stability values for zebra finch samples, calculated by NormFinder. Samples were grouped by sex and NormFinder was used to calculate expression stability values (M) for individual genes and the best combination of two genes. Genes are listed from most to least stable (lowest to highest M value).
| Tissue | Gene | M | |
|---|---|---|---|
| Brain | |||
| Best combination | GAPDH, PPIA | 0.013 | |
| Most stable overall | PPIA | 0.013 | |
| GAPDH | 0.022 | ||
| RPL32 | 0.024 | ||
| RPL4 | 0.024 | ||
| ACTB | 0.028 | ||
| HPRT | 0.032 | ||
| 18S | 0.050 | ||
| TFRC | 0.051 | ||
| UBC | 0.075 | ||
| Pituitary | |||
| Best combination | PPIA, RPL4 | 0.017 | |
| Most stable overall | PPIA | 0.023 | |
| RPL4 | 0.024 | ||
| RPL32 | 0.030 | ||
| GAPDH | 0.042 | ||
| 18S | 0.059 | ||
| HPRT | 0.065 | ||
| ACTB | 0.082 | ||
| UBC | 0.117 | ||
| TFRC | 0.120 | ||