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. Author manuscript; available in PMC: 2014 Aug 14.

Published in final edited form as: J Pharm Sci. 2012 May 11;101(8):2763–2776. doi: 10.1002/jps.23180

Remote-loaded liposomes of DSPC:PEG-DSPE:Chol (9:1:5 mol:mol:mol) were dialyzed and centrifuged for 6 min at 7500g. The final drug:lipid ratio was 0.60 mol:mol. Liposomes remaining in the supernatant were diluted in buffered saline and examined by TEM after negative staining in uranyl acetate. (A) Examination of multiple fields showed that the preparation was free of precipitated drug. Bar: 100 nm. (B) Higher-magnification image of the liposome formulation from panel (A), showing apparent electron-dense material associated with structures that appear to be liposomes. Bar: 50 nm.

Remote-loaded liposomes of DSPC:PEG-DSPE:Chol (9:1:5 mol:mol:mol) were dialyzed and centrifuged for 6 min at 7500g. The final drug:lipid ratio was 0.60 mol:mol. Liposomes remaining in the supernatant were diluted in buffered saline and examined by TEM after negative staining in uranyl acetate. (A) Examination of multiple fields showed that the preparation was free of precipitated drug. Bar: 100 nm. (B) Higher-magnification image of the liposome formulation from panel (A), showing apparent electron-dense material associated with structures that appear to be liposomes. Bar: 50 nm.