Table 1.

Selection of complexing ion for remote loading of gefitinib

Complexing ion	Gefitinib mM ±s.d.		Phospholipid mM ±s.d.		Drug:Lipid Ratio ±s.d.
	Initial†	Final	Initial	Final	Initial	Final
Citrate	2.21 ±0.23	0.87 ±0.02	10.50 ±0.30	5.55 ±0.67	0.21 ±0.03	0.16 ±0.02 *
Ammonium Sulfate	2.12 ±0.24	1.08 ±0.26	11.39 ±0.34	5.69 ±0.12	0.19 ±0.02	0.19 ±0.04
Tris/NaCl	4.59 ±1.88	0.23 ±0.01	18.35 ±0.67	7.67 ±0.29	0.25 ±0.11	0.03 ±0.002 *
Free Drug	1.51 ±0.78	0.25 ±0.01	-	-

s.d.: standard deviation

^†n=triplicate determinations for each sample

^*Decreased significantly from initial value (p<0.05)