Table 2.
Central composite design and experimental results for the biomass concentration, lipid concentration, lipid content, and GLA concentration produced by Cunninghamella bainieri 2A1 in repeated batch fermentation at 30°C for 96 h.
| Run | X 1 | X 2 | X 3 | C/N | Biomass (g/L) |
Lipid concentration (g/L) |
Lipid content (%) |
GLA (g/L) |
|---|---|---|---|---|---|---|---|---|
| 1 | 0 | 0 | +1 | 29.45 | 21.5 | 6.1 | 28.3 | 0.33 |
| 2 | +1 | −1 | −1 | 71.5 | 17.65 | 2.85 | 16.1 | 0.17 |
| 3 | +1 | −1 | +1 | 71.5 | 15.95 | 5.35 | 33.5 | 0.15 |
| 4 | 0 | 0 | 0 | 29.45 | 20.9 | 4.5 | 21.5 | 0.21 |
| 5 | 0 | +1 | 0 | 20.38 | 19.6 | 4.1 | 20.9 | 0.22 |
| 6 | −1 | −1 | −1 | 35 | 17.15 | 3.0 | 17.4 | 0.45 |
| 7 | +1 | +1 | −1 | 27.5 | 20.4 | 3.26 | 15.9 | 0.40 |
| 8 | −1 | 0 | 0 | 19.45 | 19.5 | 4.4 | 22.5 | 0.18 |
| 9 | 0 | 0 | −1 | 29.45 | 20.8 | 3.2 | 15.3 | 0.30 |
| 10 | 0 | 0 | 0 | 29.45 | 21.0 | 4.5 | 21.4 | 0.19 |
| 11 | 0 | 0 | 0 | 29.45 | 21.5 | 4.7 | 21.8 | 0.15 |
| 12 | −1 | −1 | +1 | 35 | 16.65 | 5.95 | 35.7 | 0.40 |
| 13 | 0 | 0 | 0 | 25.1 | 21.15 | 4.5 | 21.2 | 0.18 |
| 14 | +1 | +1 | +1 | 27.5 | 21.1 | 5.3 | 25.1 | 0.28 |
| 15 | −1 | +1 | −1 | 13.46 | 19.2 | 2.4 | 14.5 | 0.35 |
| 16 | 0 | −1 | 0 | 53 | 16.3 | 4.3 | 26.3 | 0.12 |
| 17 | +1 | 0 | 0 | 39.73 | 19.7 | 4.35 | 22.0 | 0.20 |
| 18 | −1 | +1 | +1 | 13.46 | 20.4 | 4.85 | 24.0 | 0.29 |
X 1: glucose concentration (g/L); X 2: ammonium tartrate (g/L); X 3: harvesting time (h); C/N: the ratio of initial carbon content (mol) to nitrogen content (mol) used in medium.