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. 2014 Jul 24;2014:918143. doi: 10.1155/2014/918143

Figure 2.

Figure 2

TcpF's sequence polymorphism. (a) SNP frequency in the tcpF locus of 68 E. faecalis UTI isolates. Sequence alignment was conducted on tcpF of E. faecalis Symbioflor 1. Black bars indicate mutations resulting in amino acid substitutions and white bars indicate silent mutations. The amino acid substitutions are G121A: Ala-Thr, C226G: Gln-Lys, C551T: Thr-Ile, and T809C: Leu-Ser. (b) Genetic structure of the tcpF locus of E. faecalis isolates. Two major rearrangements were observed in the UTI isolates E. faecalis #53 and #31. P1/P2: primer positions for the screening-PCR; P3/P4: primer positions for the sequencing-PCR.