Figure 4.

TcpF suppresses TNF-α induction. (a) E. faecalis Symbioflor 1 and its isogenic tcpF deletion mutant Symbioflor 1 ΔtcpF show identical growth rate. Fresh culture was started from overnight culture 1 : 25 in BHI at 37°C and 100 rpm. Optical density at 600 nm (OD₆₀₀) at indicated time points. (b) RAW264.7 macrophages were infected for 6 h with E. faecalis Symbioflor 1 and E. faecalis Symbioflor 1 ΔtcpF at indicated moi. NC: negative control—uninfected macrophages. Error bars represent s.d. (n = 4). Student's t-test: ∗∗∗P < 0,001; ∗∗P < 0,01.