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. 2014 Jul 24;2014:918143. doi: 10.1155/2014/918143

Figure 5.

Figure 5

Intracellular expression of TcpF impairs TLR2 dependent NFκB activation. HEK293 cells were transiently transfected with hTLR2 (50 ng), pRK5, reporter plasmids pELAM-Luc and phRL-TK, and increasing amounts of TcpF (a) or TcpC1 (b) expressing plasmids at ratios of 1 : 1, 1 : 2, 1 : 5, 1 : 10, and 1 : 31. Cells were stimulated with 0.2 μM PAM3 and incubated for 5 h. Nonstimulated and TLR2 deficient cells (TLR2-) served as controls. Error bars represent s.d. (n = 3). RLU: relative luciferase unit.