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β-arrestin2 is recruited to DOPr by SNC80, not ARM390, in sensory neurons. TG neurons from rats were pretreated with SNC80 (1 μM), ARM390 (1 μM), or vehicle (0.1% DMSO) for 30 min. Cells were rinsed and association of DOPr or TRPV1 with β-arrestin2 was assessed via co-immunoprecipitation and Western blot. A) Representative blot following IP for DOPr or TRPV1 and probe for β-arrestin2. B) Mean ± SEM from 3 independent experiments per treatment condition. **, p > 0.01 by one-way ANOVA.
