Figure 12.
Effects of treatment with 10−5 M 7-methoxycoumarin or osthole or neither (control) on bone resorption pits formed by rabbit osteoclasts at the 9th day. Bone resorption pits were stained by toluidine blue (a). Number (b), total area (c), and intensity of staining (integrated optical density or IOD) (d) of bone resorption pits were measured by Image-Pro Plus 6.0 software. Data were shown as mean ± SD of triplicate cultures. *P < 0.05, **P < 0.01 versus control; # P < 0.05, ## P < 0.01 versus 7-methoxycoumarin.