Fig. 2.

In vivo expression of a tetracycline-inducible gene in mice. Mice were derived from KH2 ES cells that carry both the R26-rtTA allele and the Flp-in TRE-AIMP3-HA allele (Beard et al., 2006). Doxycyline was administered to the mice in drinking water for 5 days (2 mg/ml, supplemented with sucrose 10 mg/ml). Thymocytes were harvested and analyzed by immunoblot with anti-HA or anti-AIMP3 antibodies. β-actin was used as a loading control. AIMP3: ARS-interacting multifunctional protein 3.