Fig. 2.

Demonstration of concentration gradient in microfluidic system using color dye solution (A) Trapping of 8 different concentration gradient of a sensitizer through a diffusive mixer by driven micropump from two reservoirs (sensitizer and medium are represented by red and yellow respectively) (B) The second trapping of 8 different concentration gradient of drug (drug was represented by blue color dye) (C) Concentration gradient of color dye was maintained 4 hours after closing valves without perfusion of reagent (D) Representative cell culture chamber with PC3 cells trapped and grown for 24 hrs.