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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Bone Miner Res. 2014 May;29(5):1170–1182. doi: 10.1002/jbmr.2125

Fig. 2. Etanercept administration to neonatal Sh3bp2KI/KI mutants improves facial swelling and loss of body weight.

Fig. 2

(A) Overall experimental procedure of the etanercept administration to neonatal mice. One-week-old wild-type (Sh3bp2+/+) and homozygous cherubism mutant (Sh3bp2KI/KI) mice were injected with PBS or etanercept (0.5 mg/kg or 25 mg/kg twice per week) for 7 weeks following genotyping at 3-5 days of age. Mice were sacrificed at 8 weeks of age for analysis. Some of the Sh3bp2KI/KI mice treated with 25 mg/kg etanercept were analyzed 8 weeks after discontinuation of etanercept treatment (16 weeks old). (B) Facial appearance of 8-week-old PBS-administered Sh3bp2KI/KI mouse and etanercept-treated Sh3bp2+/+ and Sh3bp2KI/KI mouse after 7 weeks of treatment. Blue or green arrow indicates the closed eyelids caused by skin inflammation, which is typically seen in Sh3bp2KI/KI mice around 6 weeks after birth. Red arrow points at open eyelids of a Sh3bp2KI/KI mouse treated with 25 mg/kg etanercept, demonstrating rescue of facial skin inflammation. Numbers represent percentages of Sh3bp2KI/KI mice with rescue of facial swelling. (C) Body weight changes in PBS- or etanercept-administered Sh3bp2+/+ and Sh3bp2KI/KI mice. Weights are significantly higher in etanercept-administered Sh3bp2KI/KI mice (red line) compared to PBS-administered Sh3bp2KI/KI mice (blue line) after 7-week treatment. Numbers in parentheses represent the number of the mice weighed. (D) Facial appearance of 8-week-old etanercept (25 mg/kg)-treated Sh3bp2KI/KI mouse (top) and the same mouse after 8 weeks of etanercept discontinuation (bottom). (E) Body weight changes in PBS- or etanercept-treated Sh3bp2+/+ and Sh3bp2KI/KI mice. Weight gain is arrested in etanercept-discontinued Sh3bp2KI/KI mice (red line) compared to PBS or etanercept-treated Sh3bp2+/+ mice (black line). Arrows indicate the age at etanercept discontinuation. Numbers in parentheses represent the number of the mice weighed. Error bars represent ± SEM. Asterisks represent the significant difference (p < 0.05).