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. Author manuscript; available in PMC: 2014 Aug 14.
Published in final edited form as: Sci Transl Med. 2012 Jan 25;4(118):118ra12. doi: 10.1126/scitranslmed.3003401

Fig 3. Migration of human T cells to the lamina propria of the small intestine.

Fig 3

(A) Analysis of human lymphocytes extracted from the lamina propria of the small intestine from representative animals. Dot plots on the L show live hCD45 cells as a percentage of total cells gated on scatter in a teplizumab treated mouse as compared to a hIg treated control. The R plot shows the phenotypes of these cells. Data from a single mouse representative of 11 mice is shown. (FSA=forward scatter area) (B) The number and phenotype of hCD45+ cells were determined by FACS analysis of cells isolated from the gut. In the teplizumab group there was an increase in the total count of human lymphocytes. Each dot represents a single mouse (***p < 0.0005). The phenotypes included CD4+ and CD8+ T cells. (C) Immunhistochemistry of duodenum from showing human lymphocytes in a teplizumab but not control Ig treated mice. The arrows show hCD45+ (brown) T cells located in the lamina propria of the teplizumab treated mouse (red arrow). There is no evidence of inflammation and epithelial surfaces are intact A: teplizumab (X40); B: hIg treated (X40); C: teplizumab treated (X20); D: hIg treated (X20)). Individual animals representative of 6 in each group are shown.