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. Author manuscript; available in PMC: 2014 Aug 14.
Published in final edited form as: Arthritis Rheumatol. 2014 Feb;66(2):350–356. doi: 10.1002/art.38261

Figure 2. Reduction of antigen-specific T cell pro-inflammatory cytokine production post aBMT.

Figure 2

(A) Percentage of total and memory CD4+ T cells. Spleen cells were harvested post aBMT (unmanipulated graft) and stained for FACS analysis. Left panel. Control N=11, 1 week N=5, 3 weeks N=7, 7 weeks N=9. Right panel Control N=11, 1 week N=1, 3 weeks N=5, 7 weeks N=6. Shown are the mean + SEM bars. B, C and D. T cell specific cytokine production. Spleen cells were harvested one, three and seven weeks after aBMT with unmanipulated BM. The cells were cultured in medium alone (B) or in the presence of PG (10µg/ml) (C) or anti-CD3 (1µg/ml) (D) for 96 hours. The supernatants were collected and analyzed using Multiplex Immuno Assay for IFNγ, IL-17 and TNFα production. Pooled data of two separate experiments are shown. PGIA animals without aBMT (Control) were sacrificed on different time points and data of these animals are pooled together in the graph N=17–19, PGIA+ aBMT N=3 for 1 week, N=4 for 3 weeks, N=6 for 7 weeks. Depicted are mean cytokine production + SEM values at a 2-log scale. * Indicates p-value <0.05, ** indicates p-value <0.01 and *** indicates p-value <0.001 compared to the control PGIA group calculated using Mann-Whitney U test.