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Construction of bacterial biosensor for the detection of exogenous AHLs. The bacterial biosensor is deficient in AHL production and when exogenous AHL interacts with LuxR protein, the transcription of reporter genes from LuxR-AHL regulated promoter initiated. This results in the display of specific phenotypes such as β-galactosidase activity, violacein pigmentation, bioluminescence, and green fluorescent protein production.
