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. Author manuscript; available in PMC: 2015 May 6.
Published in final edited form as: Cell Metab. 2014 May 6;19(5):767–779. doi: 10.1016/j.cmet.2014.04.009

Fig. 7. Alterations in Npy, AgRP, Pomc and Mc4r expression in Rpgrip1l+/− mice.

Fig. 7

(A) mRNA levels assessed by RT-PCR using as template total hypothalamic RNA extracted from 4 Rpgrip1l+/− and 4Rpgrip1l+/+ mice fed regular chow ad lib for 18 weeks followed by the high fat diet (HFD) for 6 days before a 24-hour fast; or 4Rpgrip1l+/− and 4Rpgrip1l+/+ littermatesfed regular chow ad lib for 18 weeks followed by HFD for a week. Food intake (B) and transcript levels (C) in 8 Rpgrip1l+/− and 9 Rpgrip1l+/+ mice fed HFD at 4-weeks of age ad lib for one week. 5 Rpgrip1l+/− and 5 Rpgrip1l+/+ mice were administered leptin intraperitoneally at the start of the dark cycle, whereas 3 Rpgrip1l+/− and 4 Rpgrip1l+/+ mice were administered saline at the same time point. All mice were sacrificed 2 hours after being injected with leptin or saline (red asterisk; Fig. 2K). Npy, AgRP and Pomc mRNA levels were higher in leptin-treated Rpgrip1l+/− compared with leptin-treated Rpgrip1l+/+ mice. Each data set is annotated with a letter to facilitate comparison. Error bars represent SEM.