Table 1.

Representative phagosomal proteins detected by non-MS methods

Protein	Host cell	Detection method	Remark/protein function	Reference
Cathepsin D	Balb/C BM-MΦ	Western	Hydrolase	[114]
Rab5	Balb/C BM-MΦ	Confocal Microscopy	Early endosome marker	[93]
Rab7	J774	Western	Late endosome marker	[70]
Transferrin	Balb/C BM-MΦ	Confocal Microscopy	Recycling endosome marker	[93]
PI3P	C57Bl/6 BM MΦ	Fluorescence microscopy	Signaling	[115]
EEA1	RAW 264.7	Microscopy	Early endosome marker	[116]
Rab10	RAW 264.7	Microscopy	Membrane transport	[116]
Rab14	RAW 264.7	Fluorescent microscopy	Phagosome maturation arrest	[100]
Rab21	RAW 264.7	Fluorescent microscopy	Early endosome	[117]
Rab22	RAW 264.7	Fluorescent microscopy	Endosomal trafficking	[117]
Cellubrevin	J774	Western	Recycling endosomes	[118]
NSF	J774	Western	Membrane fusion	[118]
SNAP	J774	Western	Membrane fusion	[118]
SNAP23	J774	Western	Plasma membrane	[118]
GDIs	J774	Western	Maintains Rab5	[119]
P38 MapK	J774	Western	Cell signaling	[119]
Rab11	J774	Western	Recylcing endosomes marker	[120]
Syntaxin 3	J774	Western	Plasma membrane	[120]
Syntaxin 8	J774	Western	Early endosome	[120]
Syntaxin 13	J774	Western	Early endosome	[120]
Syntaxin 4	J774	Microscopy	Plasma membrane	[91]
TACO	J774A.1	Western	Actin binding	[121]
CIP50	J774A.1	Western	Aids coronin	[121]
Annexin	THP-1	Western	Transport/endocytosis	[122]
PI3K	THP-1	Western	PI3P synthesis	[123]
Lamp	Human monocyte	Electron microscopy	Lysosome/phagosome marker	[81]
MHC class I	Human monocyte	Electron microscopy	Antigen presentation	[81]
MHC class II	Human monocyte	Electron microscopy	Antigen presentation	[81]
Calnexin	Human DC	Microscopy	ER chaperone	[124]
CD1	Human DC	Fluorescence microscopy	Antigen presentation	[125]