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. 2014 May 30;20(9):850–860. doi: 10.1093/molehr/gau040

Figure 2.

Figure 2

CBP and CITED4 are required for LH-target gene expression in granulosa cells. (A) Immunohistochemistry results for CBP expression in mouse ovaries at PD23 with and without PMSG/hCG treatment. Scale bar = 500 µM for ×4 images; Scale bar = 100 µM for ×20 images. (B) Chromatin immunoprecipitation (ChIP) results for CBP binding to Areg and Ptgs2 promoter regions. GCs were collected from PMSG-primed mice before and after hCG treatment for 4 h. For each sample used for ChIP assay, GCs from four mice were pooled together and lysed. (C) qRT–PCR results for relative mRNA levels of the indicated genes in cultured GCs stimulated by forskolin (10 µM)/PMA (20 nM) and with or without CITED4 and CBP overexpression. *P < 0.001 when compared with sample 1 from left. **P < 0.001 when compared with sample 2 from left. (D and E) qRT–PCR results for relative mRNA levels of the indicated genes in cultured GCs stimulated by EGF and forskolin/PMA and with or without RNAi Cited4 depletion (D) or CBP inhibitor C646 treatment (E). C646 was added to the culture medium at 30 min before For/PMA stimulation, with the final concentrations of 1, 2 or 5 µM. *P < 0.001 when compared with sample 2 from left.