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. 2014 Mar 17;21(4):417–427. doi: 10.1093/dnares/dsu007

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© The Author 2014. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — DNA gel blot analysis confirms two stable plastid genome configurations in M. truncatula ssp. tricycla ptDNA using HhaI polymorphic sites. (A) Schematic map of 2HA and R108 ptDNA with the position of DNA probes P1–P4. The site of inversion is marked by x. HhaI fragment sizes are given inside the circles. (B) Probing HhaI-digested total cellular DNA of four 2HA (H) and four R108 (R) plants with probes P1–P4. (C) Testing ptDNA genome structure in M. truncatula ssp. tricyla lines in HhaI-digested total cellular DNA using probes P1–P4. The lanes contain DNA of lines 2529, T1; 2624, T2; 761, T3; 1665, T4; GR546, T5; 765, T6; W611366, T7.