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. 2014 Aug 13;9(8):e102709. doi: 10.1371/journal.pone.0102709

Figure 6. CD4 independence of IT induced bystander activated memory CD8+ T cells persists at longer time points.

Figure 6

Control or long term CD4+ depleted C57BL/6 mice were treated with IT or PBS/rIgG (control) and harvested at Day 15 after the initiation of therapy. Bystander memory CD8+ T cells, PD-1 expression, interferon gamma production, and granzyme B production were quantified by flow cytometric analysis. Cytotoxic effector function was assayed by a redirected lysis assay. The percentage of CD8+ T cells with the CD44high NKG2D+ CD25− bystander memory phenotype in the spleen (a) and LNs (b). PD-1 expression on the memory CD8+ T cells in the spleen (c) and LNs (d). Interferon gamma production (e) and granzyme B expression (f) in splenic memory CD8+ T cells. (g) Killing function of splenocytes from long term CD4 depleted mice expressed as percentage of maximal lysis. N = 3 mice per group (*P<.05, **P<.01, ***P<.001).