Figure 2. Hematopoietic cells derived from GvHD mice are competent for hematopoietic reconstitution (A–E) Continuous transplantation.

Analyses were performed on day 14 after second transplantation. (A) Continuous HSCT: To evaluate the effects of GvHD on hematopoietic cell competency, lethally irradiated C57BL/6 (CD45.2) mice received BM cells(5×10⁶) from either BMT or GvHD recipient mice of [B6.SJL (CD45.1)→CB6F1 (CD45.1/2)] model at 14 days after first transplantation. (B) Representative flow cytometry analysis of.B cells (B220⁺), granulocytes (Gr-1⁺), and monocytes (CD11b⁺) in the recipient BM cells after continuous transplantation. (C) MNC count per tibia. (D) Percentages of B cells, granulocytes and monocytes in MNCs. (E) Counts of B cells, granulocytes and monocytes. (F–L) Competitive transplantation. Analyses were performed on day 30 after second transplantation. (F) Competitive transplantation to further evaluate the competency of hematopoietic cells in GvHD mice: 14 days after the first transplantation, BM cells (2.5×10⁶) from the transplanted mice [B6.SJL (CD45.1)→CB6F1 (CD45.1/2)] were mixed with equal amount (2.5×10⁶) of BM cells from healthy C57BL/6 mice (CD45.2), and transplanted into C57BL/6 recipients (CD45.2) after 8Gy radiotherapy. (G) Total MNC counts and CD45.1 positive cell counts per tibia. (H) Representative flow cytometry profile of HSCs (Lin⁻CD48⁻CD150⁺) analysis. (I) Absolute number of Lin⁻CD48⁻CD150⁺ cells in CD45.1 positive cell. (J) The percentage of Lin⁻CD48⁻CD150⁺ cells in CD45.1 positive cell. (K) and (L) Percentages and absolute number of B cells (B220⁺), granulocytes (Gr-1⁺), and monocytes (CD11b⁺), respectively. All tests were performed on day 30 after transplantation. Data are shown as mean ± SD and from 1 of 3 experiments with similar results. NS: no significant (n = 4, t-test).