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. 2014 Aug 13;9(8):e104698. doi: 10.1371/journal.pone.0104698

Figure 1. iTregs demethylated the Treg specific demethylated (TSDR) region and maintained Foxp3 expression.

Figure 1

(A) The methylation status of the Foxp3 TSDR of iTregs prior to and after transferring into TxA23 mice. Also shown are enTregs (CD4+Foxp3+) and conventional (CD4+Foxp3-) cells from TxA23 mice. Flow cytometric analysis of (B) the purity of enTregs and iTregs prior to transferring into TxA23 mice, (C) the percentage of Thy1.2+ transferred Tregs in the gastric mucosa, and (D) the expression of Foxp3.eGFP by the Thy1.2+ Tregs one week after transfer. Data shown represents 2 mice for the enTreg group and 3 mice for the iTreg group from two independent experiments.