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. 2014 Aug 13;9(8):e104698. doi: 10.1371/journal.pone.0104698

Figure 4. Comparison of cytokines and transcription factors expressed by enTregs and iTregs.

Figure 4

mRNA was isolated from enTregs and iTregs that were transferred into TxA23 mice, analyzed by qRT-PCR and normalized to mRNA isolated from CD4+Foxp3- T cells from BALB/c mice. (A) Comparison of expression levels of cytokines IL-35 (Ebi3 and Il12p35) and Tgfβ1. (B) Comparison of expression levels of transcription factors Irf4 and Tbx21 (Tbet). Data shown as mean ± SEM from 4-6 independent experiments and analyzed using a Mann–Whitney U test. *p<0.05.